%0 Journal Article
%T The roles of the Na+/K+©\ATPase, NKCC, and K+ channels in regulating local sweating and cutaneous blood flow during exercise in humans in£¿vivo
%A Glen P. Kenny
%A Jeffrey C. Louie
%A Naoto Fujii
%A Robert D. Meade
%J Archive of "Physiological Reports".
%D 2016
%R 10.14814/phy2.13024
%X Na+/K+©\ATPase has been shown to regulate the sweating and cutaneous vascular responses during exercise; however, similar studies have not been conducted to assess the roles of the Na©\K©\2Cl co©\transporter (NKCC) and K+ channels. Additionally, it remains to be determined if these mechanisms underpinning the heat loss responses differ with exercise intensity. Eleven young (24 ¡À 4 years) males performed three 30©\min semirecumbent cycling bouts at low (30% VO 2peak), moderate (50% VO 2peak), and high (70% VO 2peak) intensity, respectively, each separated by 20©\min recovery periods. Using intradermal microdialysis, four forearm skin sites were continuously perfused with either: (1) lactated Ringer solution (Control); (2) 6 mmol¡€L£¿1 ouabain (Na+/K+©\ATPase inhibitor); (3) 10 mmol¡€L£¿1 bumetanide (NKCC inhibitor); or (4) 50 mmol¡€L£¿1 BaCl2 (nonspecific K+ channel inhibitor); sites at which we assessed local sweat rate (LSR) and cutaneous vascular conductance (CVC). Inhibition of Na+/K+©\ATPase attenuated LSR compared to Control during the moderate and high©\intensity exercise bouts (both P £¿ 0.01), whereas attenuations with NKCC and K+ channel inhibition were only apparent during the high©\intensity exercise bout (both P ¡Ü 0.05). Na+/K+©\ATPase inhibition augmented CVC during all exercise intensities (all P £¿ 0.01), whereas CVC was greater with NKCC inhibition during the low©\intensity exercise only (P £¿ 0.01) and attenuated with K+ channel inhibition during the moderate and high©\intensity exercise conditions (both P £¿ 0.01). We show that Na+/K+©\ATPase, NKCC and K+ channels all contribute to the regulation of sweating and cutaneous blood flow but their influence is dependent on the intensity of dynamic exercise
%K Exercise
%K heat loss
%K K+ channels
%K microcirculation
%K Na+/K+©\ATPase
%K NKCC
%K sweat gland
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358008/