%0 Journal Article
%T Post©\MI treatment with G©\CSF and EPO©\liposome with SLX repairs infarcted myocardium through EPCs mobilization and activation of prosurvival signals in rabbits
%A Atsushi Mikami
%A Hiromitsu Kanamori
%A Kazuhiko Nishigaki
%A Masanori Kawasaki
%A Noriko Endo
%A Shingo Minatoguchi
%A Shinya Minatoguchi
%A Yoshihisa Yamada
%J Archive of "Pharmacology Research & Perspectives".
%D 2019
%R 10.1002/prp2.451
%X We investigated whether combination therapy of G©\CSF and erythropoietin (EPO)©\liposome with Siaryl Lewis X (SLX) is more cardioprotective than G©\CSF or EPO©\liposome with SLX alone. For the purpose of generating myocardial infarction (MI), rabbits underwent 30 minutes of coronary occlusion and 14 days of reperfusion. We administered saline (control group, i.v.,), G©\CSF (G group, 10 ¦Ìg/kg/day ¡Á 5 days, i.c., starting at 24 hours after reperfusion), EPO©\liposome with SLX (LE group, i.v., 2500 IU/kg EPO containing liposome with SLX, immediately after reperfusion), and G©\CSF + EPO©\liposome with SLX (LE + G group) to the rabbits. The MI size was the smallest in the LE+G group (14.7 ¡À 0.8%), and smaller in the G group (22.4 ¡À 1.5%) and LE group (18.5 ¡À 1.1%) than in the control group (27.8 ¡À 1.5%). Compared with the control group, the cardiac function and remodeling of the G, LE, and LE + G groups were improved, and LE + G group tended to show the best improvement. The number of CD31©\positive microvessels was the greatest in the LE + G group, greater in the G and LE groups than in the control group. Higher expressions of phosphorylated (p)©\Akt and p©\ERK were observed in the ischemic area of the LE and LE + G groups. The number of CD34+/CXCR4+ cells was significantly higher in the G and LE + G groups. The cardiac SDF©\1 was more expressed in the G and LE + G groups. In conclusion, Post©\MI combination therapy with G©\CSF and EPO©\liposome with SLX is more cardioprotective than G©\CSF or EPO©\liposome with SLX alone through EPCs mobilization, neovascularization, and activation of prosurvival signals
%K EPC
%K erythropoietin
%K G©\CSF
%K infarct size
%K liposome
%K signal transduction
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302719/