%0 Journal Article %T Spatially-localized bench-top X-ray scattering reveals tissue-specific microfibril orientation in Moso bamboo %A Aki Kallonen %A Heikki Suhonen %A Kirsi Svedstr£żm %A Lorna J. Gibson %A Patrick G. Dixon %A Patrik Ahvenainen %J Archive of "Plant Methods". %D 2017 %R 10.1186/s13007-016-0155-1 %X Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple length scales has great potential to reveal novel information. This article presents one such method with an application to the bamboo culm wall. Moso (Phyllostachys edulis) bamboo is a commercially important bamboo species. At the cellular level, bamboo culm wall consists of vascular bundles embedded in a parenchyma cell tissue matrix. The microfibril angle (MFA) in the bamboo cell wall is related to its macroscopic longitudinal stiffness and strength and can be determined at the nanoscale with wide-angle X-ray scattering (WAXS). Combining WAXS with X-ray microtomography (XMT) allows tissue-specific study of the bamboo culm without invasive chemical treatment %K Bamboo parenchyma %K Phyllostachys edulis %K Wide-angle X-ray scattering %K X-ray microtomography %K Microfibril angle %K Spatially-localized scattering %K Diffraction-contrast tomography %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223584/