%0 Journal Article
%T High frequency plant regeneration from leaf culture of Neolamarckia cadamba
%A Deng Zhang
%A Jingjian Li
%A Kunxi Ouyang
%A Xiaoyang Chen
%J Archive of "Plant Biotechnology".
%D 2019
%R 10.5511/plantbiotechnology.18.1119a
%X Neolamarckia cadamba is a miracle tree species with considerable economic potential uses as a timber wood, woody forage and traditional medicine resource. The present study aimed to establish a highly efficient and robust protocol of plant regeneration for N. cadamba. Greenish callus was induced from very young leaf explants of sterile in vitro plantlets cultured on Murashige and Skoog¡¯s (MS) medium supplemented with 3£¿mg£¿l£¿1 thidiazuron (TDZ), 0.1£¿mg£¿l£¿1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05£¿mg£¿l£¿1 ¦Á-naphthaleneacetic acid (NAA). The callus could differentiate into nodular embryogenic structures or adventitious shoots, and these two regeneration pathways often occurred in the same callus clumps. The micro-shoots developed roots in MS supplemented with 0.05£¿mg£¿l£¿1 NAA and 0.05£¿mg£¿l£¿1 indole-3-butyric acid (IBA), while the nodular embryogenic structures germinated directly and developed into plantlets on induction medium contained with 0.5£¿mg£¿l£¿1 (or 1£¿mg£¿l£¿1) 6-benzyladenine (6-BA) and 0.05£¿mg£¿l£¿1 NAA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 92.0% survival. This regeneration protocol can be used in large scale cultivation needs and may be useful for future genetic modifications of N. cadamba
%K adventitious shoots
%K In vitro culture
%K leaf explants
%K Neolamarckia cadamba
%K plant regeneration
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566011/