%0 Journal Article
%T Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model
%A Adrianna Jensen
%A Chao Wang
%A Nils A. Loewen
%A Ralitsa T. Loewen
%A Richard A. Bilonick
%A Susannah Waxman
%A Yalong Dang
%J Archive of "PeerJ".
%D 2017
%R 10.7717/peerj.3629
%X The trabecular meshwork (TM) is the primary substrate of outflow resistance in glaucomatous eyes. Repopulating diseased TM with fresh, functional TM cells might be a viable therapeutic approach. Decellularized TM scaffolds have previously been produced by ablating cells with suicide gene therapy or saponin, which risks incomplete cell removal or dissolution of the extracellular matrix, respectively. We hypothesized that improved trabecular meshwork cell ablation would result from freeze-thaw cycles compared to chemical treatment
%K Trabecular meshwork
%K Decellularization
%K Glaucoma
%K Pig eyes
%K Ablation
%K Intraocular pressure
%K Freeze-thaw
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560227/