%0 Journal Article
%T 5¡ä-non-transcribed flanking region and 5¡ä-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in Arabidopsis roots
%A Akiko Maruyama-Nakashita
%A Akiko Suyama
%A Hideki Takahashi
%J Archive of "Plant Biotechnology".
%D 2017
%R 10.5511/plantbiotechnology.16.1226a
%X Plants increase sulfate uptake activity under sulfur deficiency (£¿S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under £¿S. Though it is known that transcript levels of SULTR1;2 increase under £¿S largely due to the function of 5¡ä-upstream region, contributions of 5¡ä-non-transcribed flanking region and 5¡ä-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5¡äUTR of SULTR1;2 in £¿S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5¡ä-upstream region of SULTR1;2 with (PL2160) or without (PL2160¦¤UTR) the 154-bp 5¡äUTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under £¿S relative to +S regardless of presence of the 5¡äUTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5¡äUTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160¦¤UTR. These results indicate an essential role of the 5¡ä-non-transcribed flanking region of SULTR1;2 at positions £¿2160 to £¿155 in £¿S-responsive transcriptional regulation
%K sulfate transporter
%K SULTR1
%K 2
%K £¿S-inducible expression
%K translation
%K 5¡äUTR
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543697/