%0 Journal Article
%T Acute treadmill exercise discriminately improves the skeletal muscle insulin©\stimulated growth signaling responses in mice lacking REDD1
%A Bradley S. Gordon
%A Cory M. Dungan
%A David L. Williamson
%J Archive of "Physiological Reports".
%D 2019
%R 10.14814/phy2.14011
%X A loss of the regulated in development and DNA damage 1 (REDD1) hyperactivates mechanistic Target of Rapamycin Complex 1 (mTORC1) reducing insulin©\stimulated insulin signaling, which could provide insight into mechanisms of insulin resistance. Although aerobic exercise acutely inhibits mTORC1 signaling, improvements in insulin©\stimulated signaling are exhibited. The goal of this study was to determine if a single bout of treadmill exercise was sufficient to improve insulin signaling in mice lacking REDD1. REDD1 wildtype (WT) and REDD1 knockout (KO) mice were acutely exercised on a treadmill (30 min, 20 m/min, 5% grade). A within animal noninsulin©\to©\insulin©\stimulated percent change in skeletal muscle insulin©\stimulated kinases (IRS©\1, ERK1/2, Akt), growth signaling activation (4E©\BP1, S6K1), and markers of growth repression (REDD1, AMPK, FOXO1/3A) was examined, following no exercise control or an acute bout of exercise. Unlike REDD1 KO mice, REDD1 WT mice exhibited an increase (P < 0.05) in REDD1 following treadmill exercise. However, both REDD1 WT and KO mice exhibited an increase (P < 0.05) AMPK phosphorylation, and a subsequent reduction (P < 0.05) in mTORC1 signaling after the exercise bout versus nonexercising WT or KO mice. Exercise increased (P < 0.05) the noninsulin©\to©\insulin©\stimulated percent change phosphorylation of mTORC1, ERK1/2, IRS©\1, and Akt on S473 in REDD1 KO mice when compared to nonexercised KO mice. However, there was no change in the noninsulin©\to©\insulin©\stimulated percent change activation of Akt on T308 and FOXO1/3A in the KO when compared to WT or KO mouse muscle after exercise. Our data show that a bout of treadmill exercise discriminately improves insulin©\stimulated signaling in the absence of REDD1
%K Aerobic exercise
%K FOXO
%K insulin signaling
%K mTORC1
%K REDD1
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383112/