%0 Journal Article
%T UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts
%A Ewald Janssens
%A Filip Rolland
%A Koen Geuten
%A Kurt Boonen
%A Liliane Schoofs
%A Piero Ferrari
%A Vera van Noort
%A Zhicheng Zhang
%J Archive of "Plant Methods".
%D 2016
%R 10.1186/s13007-016-0142-6
%X The complexity of RNA regulation is one of the current frontiers in animal and plant molecular biology research. RNA-binding proteins (RBPs) are characteristically involved in post-transcriptional gene regulation through interaction with RNA. Recently, the mRNA-bound proteome of mammalian cell lines has been successfully cataloged using a new method called interactome capture. This method relies on UV crosslinking of proteins to RNA, purifying the mRNA using complementary oligo-dT beads and identifying the crosslinked proteins using mass spectrometry. We describe here an optimized system of mRNA interactome capture for Arabidopsis thaliana leaf mesophyll protoplasts, a cell type often used in functional cellular assays
%K Messenger RNA-binding proteins
%K Messenger ribonucleoprotein complexes
%K Arabidopsis thaliana leaf mesophyll protoplasts
%K In vivo UV crosslinking
%K mRNA-bound proteome
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093948/