%0 Journal Article
%T Seed Dormancy Involves a Transcriptional Program That Supports Early Plastid Functionality during Imbibition
%A Alberto Gianinetti
%A Antonella Zechini
%A Chiara Biselli
%A Franca Finocchiaro
%A Giampiero Val¨¨
%A Luigi Cattivelli
%A Paolo Bagnaresi
%A Primetta Faccioli
%J Archive of "Plants".
%D 2018
%R 10.3390/plants7020035
%X Red rice fully dormant seeds do not germinate even under favorable germination conditions. In several species, including rice, seed dormancy can be removed by dry-afterripening (warm storage); thus, dormant and non-dormant seeds can be compared for the same genotype. A weedy (red) rice genotype with strong dormancy was used for mRNA expression profiling, by RNA-Seq, of dormant and non-dormant dehulled caryopses (here addressed as seeds) at two temperatures (30 ˇăC and 10 ˇăC) and two durations of incubation in water (8 h and 8 days). Aim of the study was to highlight the differences in the transcriptome of dormant and non-dormant imbibed seeds. Transcript data suggested important differences between these seeds (at least, as inferred by expression-based metabolism reconstruction): dry-afterripening seems to impose a respiratory impairment onto non-dormant seeds, thus glycolysis is deduced to be preferentially directed to alcoholic fermentation in non-dormant seeds but to alanine production in dormant ones; phosphoenolpyruvate carboxykinase, pyruvate phosphate dikinase and alanine aminotransferase pathways appear to have an important gluconeogenetic role associated with the restoration of plastid functions in the dormant seed following imbibition; correspondingly, co-expression analysis pointed out a commitment to guarantee plastid functionality in dormant seeds. At 8 h of imbibition, as inferred by gene expression, dormant seeds appear to preferentially use carbon and nitrogen resources for biosynthetic processes in the plastid, including starch and proanthocyanidins accumulation. Chromatin modification appears to be a possible mechanism involved in the transition from dormancy to germination. Non-dormant seeds show higher expression of genes related to cell wall modification, suggesting they prepare for acrospire/radicle elongation
%K dry-afterripening
%K weedy rice
%K Oryza sativa
%K dormancy
%K germination
%K transcriptome
%K plastid
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026906/