%0 Journal Article
%T Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
%A Amanda Hopes
%A Sophien Kamoun
%A Thomas Mock
%A Vladimir Nekrasov
%J Archive of "Plant Methods".
%D 2016
%R 10.1186/s13007-016-0148-0
%X CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of Thalassiosira pseudonana, a model centric diatom with both ecological significance and high biotechnological potential, using CRISPR-Cas
%K CRISPR-Cas
%K Diatom
%K Genome editing
%K Urease
%K Golden Gate
%K Thalassiosira pseudonana
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121945/