%0 Journal Article %T A rapid and cost-effective fluorescence detection in tube (FDIT) method to analyze protein phosphorylation %A Jin-Ying Gou %A Xiao Jin %J Archive of "Plant Methods". %D 2016 %R 10.1186/s13007-016-0143-5 %X Protein phosphorylation is one of the most important post-translational modifications catalyzed by protein kinases in living organisms. The advance of genome sequencing provided the information of protein kinase families in many organisms, including both model and non-model plants. The development of proteomics technologies also enabled scientists to efficiently reveal a large number of protein phosphorylations of an organism. However, kinases and phosphorylation targets are still to be connected to illustrate the complicated network in life %K Protein kinase reaction %K Fluorescence %K Pro-Q£¿ diamond %K Wheat Kinase START 1 %K Thylakoid ascorbate peroxidase %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094037/