%0 Journal Article
%T Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.
%A Andrew S. Fister
%A Emily E. Helliwell
%A Mark J. Guiltinan
%A Siela N. Maximova
%A Yufan Zhang
%A Zi Shi
%J Archive of "Plant Methods".
%D 2016
%R 10.1186/s13007-016-0119-5
%X Theobroma cacao L., the source of cocoa, is a crop of significant economic value around the world. To facilitate the study of gene function in cacao we have developed a rapid Agrobacterium-mediated transient genetic transformation protocol. Here we present a detailed methodology for our transformation assay, as well as an assay for inoculation of cacao leaves with pathogens
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788949/