%0 Journal Article
%T A modular toolbox for gRNA¨CCas9 genome engineering in plants based on the GoldenBraid standard
%A Antonio Granell
%A Asun Fernandez-del-Carmen
%A Diego Orzaez
%A Joan Miquel Bernab¨¦-Orts
%A Jose Blanca
%A Marta Vazquez-Vilar
%A Pello Ziarsolo
%J Archive of "Plant Methods".
%D 2016
%R 10.1186/s13007-016-0101-2
%X The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the construction of transcriptional gene circuits, many of which depend on the technical ability to compose and transfer complex synthetic instructions into the plant cell. The engineering principles of standardization and modularity applied to DNA cloning are impacting plant genetic engineering, by increasing multigene assembly efficiency and by fostering the exchange of well-defined physical DNA parts with precise functional information
%K Plant gene editing
%K Plant gene activation
%K Plant gene repression
%K CRISPR/Cas9
%K gRNAs
%K Multigenic assemblies
%K GoldenBraid
%K Luciferase/renilla assay
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736081/