%0 Journal Article
%T The Copper Amine Oxidase AtCuAO¦Ä Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis
%A Alessandra Cona
%A Andrea Carucci
%A Ilaria Fraudentali
%A Paraskevi Tavladoraki
%A Renato A. Rodrigues-Pousada
%A Riccardo Angelini
%A Sandip A. Ghuge
%J Archive of "Plants".
%D 2019
%R 10.3390/plants8060183
%X Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAO¦Ä (AtCuAO¦Ä; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAO¦Ä is up-regulated by ABA and that two Atcuao¦Ä T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAO¦Ä over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2O2 production compared to WT plants. Our data suggest that AtCuAO¦Ä is involved in the H2O2 production related to ABA-induced stomatal closure
%K copper amine oxidases
%K H2O2
%K ROS
%K polyamines
%K ABA
%K stomatal closure
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630932/