%0 Journal Article
%T Hemoglobin as a probe for estimation of nitric oxide emission from plant tissues
%A Neha Singh
%A Satish C. Bhatla
%J Archive of "Plant Methods".
%D 2019
%R 10.1186/s13007-019-0425-9
%X a Mechanism of action of MnIP-Cu, a novel probe for detection of NO in plant systems. b Quenching of NO released from seedling roots by exogenous hemoglobin (Hb) in a concentration (Hb)-dependent manner. NO released in solution from seedling roots bathed in varied concentrations of Hb was estimated using NO specific probe MnIP-Cu ex. 330 nm, em. 460 nm. c Conversion of oxyhemoglobin (HbO2) to methemoglobin in the presence of NO. d Absorbance peak for formation of methemoglobin in the presence of NO released from seedling roots in the solution bathed in varying concentrations of hemoglobin (250 ¦ÌM to 3 mM) separately. e Increase in methemoglobin formation as a consequence of NO release from seedling roots in the bathing solution containing variable concentrations of hemoglobin (Hb). Note: Lower dosage of Hb in solution is insufficient in quenching NO from seedling root
%K Hemoglobin
%K Nitric oxide
%K NO scavenger
%K Methemoglobin
%K MnIP-Cu
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480594/