%0 Journal Article
%T Evaluation of <i>In-Vitro</i> Anti-Tuberculosis Activity of  <i>Tetrapleura tetraptera</i> Crude and Fractions on Multidrug Resistant <i>Mycobacterium tuberculosis</i>
%A Kasim Salihu Izebe
%A Kolo Ibrahim
%A Josiah Ademola Onaolapo
%A Peters Oladosu
%A Yakubu Ya¡¯aba
%A Moses Njoku
%A Mohammed Busu Shehu
%A Mercy Ezeunala
%A Yakubu Kokori Ibrahim
%J Journal of Tuberculosis Research
%P 165-176
%@ 2329-8448
%D 2020
%I Scientific Research Publishing
%R 10.4236/jtr.2020.83015
%X The management, control and elimination of tuberculosis (TB) have been
difficult with the advent of HIV and cases of multidrug resistant (MDR-TB)
tuberculosis. The cases of multidrug
resistance to rifampicin and isoniazid pose greater challenges on first line and second line drugs to eliminate
TB. The study is aimed at establishing anti-tuberculosis activity of <i>Tetrapleura
tetraptera</i> against <i>Mycobacterium
tuberculosis</i> and MDR-TB and the phytochemical present. The leaves of <i>Tetrapleura tetraptera </i>were collected, weighed, dried and pulverized to
powder. The pulverized leaves of <i>Tetrapleura tetraptera </i>were subjected to 70% methanol extraction and
screened for phytochemical. The crude extract was further purified into
fractions using silica gel and thin layer chromatography techniques.<i> M.
tuberculosis</i> and MDR-TB were
obtained from positive acid fast bacilli sputa of TB patients and
confirmed using GeneXpert to
differentiate genotypic drug susceptible <i>M. tuberculosis</i> and MDR-TB. The sputa
were digested using sodium hydroxide-cysteine technique and cultured in
Middlebrook 7H9. The crude extract and fractions were screened for
anti-tuberculosis activity using tetrazolium microtitre plate assay. The
results showed that <i>Tetrapleura tetraptera crude </i>had activities against <i>M. tuberculosis</i> at 7.4 ¡À 0 mg/ml and 27.5 ¡À 0 mg/ml for MDR-TB. One of the fractions inhibited the growth of <i>M.
tuberculosis </i>at 0.24 ¡À 0 mg/ml and MDR-TB at 0.89 ¡À 0 mg/ml. The phytochemical screened includes tannins,
alkaloids, saponins, flavonoids, phenols and resins. <i>T. tetraptra </i>possesses
anti-tuberculosis potential at low concentration on MDR-TB and can be a lead compound in drug development for the treatment of
tuberculosis and multidrug resistant tuberculosis.
%K Anti-Tuberculosis
%K &lt
%K i&gt
%K Tetrapleura tetraptera&lt
%K /i&gt
%K Tetrazolium Assay
%K Tuberculosis
%K Phyto-Chemical
%K MDR-TB
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=102656