%0 Journal Article
%T Differential MicroRNA expression following headİ\down tilt bed rest: implications for cardiovascular responses to microgravity
%A Carl J. Ade
%A Debra A. Bemben
%J Archive of "Physiological Reports".
%D 2019
%R 10.14814/phy2.14061
%X Headİ\down tilt bedrest (HDBR), an analog of spaceflight, elicits changes in cardiovascular function that adversely affect astronaut performance. It is therefore fundamental to elucidate the molecular regulators of these changes. Study aim was to determine if cardiovascularİ\related circulating microRNA (miRNA) are altered following HDBR and if they relate to changes in cardiac function and peak aerobic capacity. Eleven participants completed 30İ\days HDBR at an ambient CO 2 of 0.5% (replicate the inİ\flight CO 2 levels). Blood samples were obtained 3 days (BDCİ\3) prior to and immediately (R + 0) following HDBR. 44İ\targeted circulating miRNAs (cİ\miRNA) identified from published roles in cardiovascular structure/function were analyzed via RTİ\qPCR. Resting stroke volume was evaluated via ultrasonography. Peak oxygen uptake ( V¨BO2peak ) was determined using a graded exercise test on an electronically braked cycle ergometer. Ten cardiovascularİ\related miRNA were significantly increased following HDBR. The differentially expressed cİ\miRNA were grouped into clusters according to their expression profile. Cluster A included cİ\miRNA that have been identified as regulators of cardiac function and hypertrophy (cİ\miRNAİ\133), atrial fibrillation and mitochondrial function (cİ\miRNAİ\1), skeletal muscle atrophy (cİ\miRNAİ\1), and vascular control (cİ\miRNAİ\155). Cluster B contained cİ\miRNA identified as regulators of cardiac hypertrophy (cİ\miRNAİ\30, İ\15), fibrosis (cİ\miRNAİ\22, İ\18), mitochondrial function (miRNAİ\181), and aerobic capacity (cİ\miRNAİ\20a). Following HDBR resting stroke volume was decreased and correlated with changes in cİ\miRNAİ\378a and İ\18a. V¨BO2peak was decreased and correlated with changes cİ\miRNAİ\133. In conclusion, we found that HDBR induced a distinct and specific cardiovascularİ\related miRNA response, which were associated with changes in cardiac function and peak aerobic capacity
%K Bed rest
%K cardiovascular
%K circulating microRNA
%K cİ\miRNA
%K microgravity
%K MicroRNA
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513770/