%0 Journal Article
%T Effects of solid©\phase extraction of plasma in measuring gut metabolic hormones in fasted and fed blood of lean and diet©\induced obese rats
%A Alvin Haver
%A Bettye Apenteng
%A Craig Lanio
%A Krista Anders
%A Roger Reidelberger
%J Archive of "Physiological Reports".
%D 2016
%R 10.14814/phy2.12800
%X Glucagon©\like peptide©\1 (GLP©\1), peptide YY (3©\36) [PYY(3©\36)], amylin, ghrelin, insulin, and leptin are thought to act as hormonal signals from periphery to brain to control food intake. Here, we determined the effects of solid©\phase extraction of plasma in measuring these hormones in blood of lean and diet©\induced obese rats. Individual enzyme©\linked immunoassays and a multiplex assay were used to measure active GLP©\1, total PYY, active amylin, active ghrelin, insulin, leptin, and total GIP in response to (1) addition of known amounts of the peptides to lean and obese plasma, (2) a large meal in lean and obese rats, and (3) intravenous infusions of anorexigenic doses of GLP©\1, PYY(3©\36), amylin, and leptin in lean rats. Extraction of lean and obese plasma prior to assays produced consistent recoveries across assays for GLP©\1, PYY, amylin, ghrelin, and insulin, reflecting losses inherent to the extraction procedure. Plasma extraction prior to assays generally revealed larger meal©\induced changes in plasma GLP©\1, PYY, amylin, ghrelin, and insulin in lean and obese rats. Plasma extraction and the multiplex assay were used to compare plasma levels of GLP©\1, PYY, and amylin after a large meal with plasma levels produced by IV infusions of anorexigenic doses of GLP©\1, PYY(3©\36), and amylin. Infusions produced dose©\dependent increases in plasma peptide levels, which were well above their postprandial levels. These results do not support the hypothesis that postprandial plasma levels of GLP©\1, PYY(3©\36), and amylin are sufficient to decrease food intake by an endocrine mechanism
%K Amylin
%K ghrelin
%K GLP©\1
%K intravenous infusion
%K PYY(3©\36)
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886168/