%0 Journal Article
%T The effect of polyphenols on cytokine and granulocyte response to resistance exercise
%A Adam R. Jajtner
%A Alyssa N. Varanoske
%A David D. Church
%A David H. Fukuda
%A Jay R. Hoffman
%A Jeffrey R. Stout
%A Jeremy R. Townsend
%A Kelli A. Herrlinger
%A Kyle S. Beyer
%A Leonardo P. Oliveira
%A Shlomit Radom©\Aizik
%J Archive of "Physiological Reports".
%D 2016
%R 10.14814/phy2.13058
%X This study examined the effect of resistance exercise on the production, recruitment, percentage, and adhesion characteristics of granulocytes with and without polyphenol (PPB) supplementation. Thirty©\eight untrained men were randomized into three groups: PPB (n = 13, 21.8 ¡À 2.5 years, 171.2 ¡À 5.5 cm, 71.2 ¡À 8.2 kg), placebo (PL; n = 15, 21.6 ¡À 2.5 years, 176.5 ¡À 4.9 cm, 84.0 ¡À 15.7 kg), or control (CON; n = 10, 23.3 ¡À 4.3 years, 173.7 ¡À 12.6 cm, 77.3 ¡À 16.3 kg). Blood samples were obtained pre (PRE), immediately (IP), 1 h (1H), 5 h (5H), 24 h (24H), 48 h (48H), and 96 h (96H) postresistance exercise (PPB/PL) or rest (CON). Fine©\needle biopsies were obtained from the vastus lateralis at PRE, 1H, 5H, and 48H. Plasma concentrations and intramuscular content of interleukin©\8 (IL©\8), granulocyte (G©\CSF), and granulocyte¨Cmacrophage colony stimulating factor (GM©\CSF) were analyzed via multiplex assays. Changes in relative number of circulating granulocytes and adhesion receptor (CD11b) were assessed using flow cytometry. Intramuscular IL©\8 was significantly elevated at 1H, 5H, and 48H (P < 0.001). Area under the curve analysis indicated a greater intramuscular IL©\8 content in PL than PPB (P = 0.011). Across groups, circulating G©\CSF was elevated from PRE at IP (P < 0.001), 1H (P = 0.011), and 5H (P = 0.025), while GM©\CSF was elevated at IP (P < 0.001) and 1H (P = 0.007). Relative number of granulocytes was elevated at 1H (P < 0.001), 5H (P < 0.001), and 24H (P = 0.005, P = 0.006) in PPB and PL, respectively. Across groups, granulocyte CD11b expression was upregulated from PRE to IP (P < 0.001) and 1H (P = 0.015). Results indicated an increase in circulating CD11b on granulocytes, and IL©\8 within the muscle following intense resistance exercise. Polyphenol supplementation may attenuate the IL©\8 response, however, did not affect granulocyte percentage and adhesion molecule expression in peripheral blood following resistance exercise
%K CD11b/CD18
%K exercise immunology
%K granulocyte colony stimulating factor
%K granulocyte¨Cmacrophage colony stimulating factor
%K inflammation
%K interleukin©\8
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5210375/