%0 Journal Article
%T Free radical scavenging decreases endothelinİ\1 excretion and glomerular albumin permeability during type 1 diabetes
%A Carmen De Miguel
%A David I. Stevens
%A David M. Pollock
%A Jennifer S. Pollock
%A Mohamed A. Saleh
%A Pamela K. Carmines
%J Archive of "Physiological Reports".
%D 2016
%R 10.14814/phy2.13055
%X Increased renal endothelinİ\1 (ETİ\1) production and an ETA receptorİ\dependent increase in glomerular albumin permeability (Palb) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1Dİ\induced oxidative stress contributes to renal ETİ\1 production and glomerular Palb. Male rats with streptozotocinİ\induced T1D were provided free access to drinking water without additives (T1D rats) or containing the free radical scavenger tempol (1 mmol/L; T1D+Tempol). After 3 weeks, T1D+Tempol rats displayed lower urinary excretion of thiobarbituric acid reactive substances and glomerular superoxide production (dihydroethidium staining) compared to T1D rats. Urinary ETİ\1 excretion and inner medullary (but not cortical or outer medullary) preproİ\ETİ\1 mRNA expression were lower in the T1D+Tempol group than in the T1D group. Palb, measured as the change in volume of isolated glomeruli upon exposure to oncotic gradients of albumin, was significantly lower in the T1D+Tempol group than in the T1D group. Tempol treatment did not alter protein excretion or creatinine clearance. These data support the postulate that oxidative stress contributes to glomerular Palb and renal ETİ\1 production during the early phase of type 1 diabetes
%K Diabetes
%K ETİ\1
%K glomerular permeability
%K tempol
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5210388/