%0 Journal Article
%T Acute administration of interleukinŠ\6 does not increase secretion of glucagonŠ\like peptideŠ\1 in mice
%A Berit Svendsen
%A Bolette Hartmann
%A Carolyn F. Deacon
%A Charlotte B. Christiansen
%A Emilie BalkŠ\MŖŋller
%A Jens J. Holst
%A Nicolai J. Wewer Albrechtsen
%A Rune E. Kuhre
%A Sara J. Lind
%A Thomas MandrupŠ\Poulsen
%A Tina Dahlby
%J Archive of "Physiological Reports".
%D 2018
%R 10.14814/phy2.13788
%X Interleukin 6 (ILŠ\6) is a cytokine secreted from skeletal muscle in response to exercise which, based on animal and cell studies, has been suggested to contribute to glucose metabolism by increasing secretion of the incretin hormone glucagonŠ\like peptideŠ\1 (GLPŠ\1) and affecting secretion of insulin and glucagon from the pancreatic islets. We investigated the effect of ILŠ\6 on GLPŠ\1 secretion in GLPŠ\1 producing cells (GLUTag) and using the perfused mouse small intestine (harboring GLPŠ\1 producing cells). Furthermore, the direct effect of ILŠ\6 on insulin and glucagon secretion was studied using isolated perfused mouse pancreas. Incubating GLUTag cells with 1000 ng/mL of ILŠ\6 for 2 h did not significantly increase secretion of GLPŠ\1 whereas 10 mmol/L glucose (positive control) did. Similarly, ILŠ\6 (100 ng/mL) had no effect on GLPŠ\1 secretion from perfused mouse small intestine whereas bombesin (positive control) increased secretion. Finally, administering ILŠ\6 (100 ng/mL) to perfused mouse pancreases did not significantly increase insulin or glucagon secretion regardless of perfusate glucose levels (3.5 vs. 12 mmol/L glucose). Acute effects of ILŠ\6 therefore do not seem to include a stimulatory effect on GLPŠ\1 secretion in mice
%K GLPŠ\1
%K glucagon
%K glucose
%K ILŠ\6
%K insulin
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6035335/