%0 Journal Article
%T Paternal high©\fat diet enhances offspring whole©\body insulin sensitivity and skeletal muscle insulin signaling early in life
%A Brian C. Clark
%A Felicia V. Nowak
%A Gunjan Saxena
%A Leslie A. Consitt
%A Max Friedlander
%A Yizhu Zhang
%A Yuriy Slyvka
%J Archive of "Physiological Reports".
%D 2018
%R 10.14814/phy2.13583
%X Evidence suggests that paternal diet can predispose offspring to metabolic dysfunction. Despite this knowledge, little is known regarding the effects of paternal high©\fat feeding on offspring insulin sensitivity. The purpose of this study was to investigate for the first time the effects of paternal high©\fat feeding on whole©\body and skeletal muscle insulin action in young and adult offspring. At 4 weeks of age, founder C57BL6/N males (F0) were fed a high©\fat diet or control diet for 12 weeks and then bred with females on a control diet. Offspring (F1) were euthanized at 6 weeks, 6 months, or 12 months and insulin©\stimulated insulin signaling was measured ex vivo in isolated soleus muscle. At 6 weeks of age, paternal high fat offspring (HFO) had enhanced whole©\body insulin sensitivity (35%, P < 0.05), as well as, increased insulin©\stimulated skeletal muscle phosphorylation of Akt threonine 308 (70%, P < 0.05) and AS160 threonine 642 (80%, P < 0.05) compared to paternal control fed offspring (CFO), despite both offspring groups consuming standard chow. At 6 months of age, HFO had increased percent body fat compared to CFO (74%, P < 0.005) and whole©\body and skeletal muscle insulin signaling normalized to CFO. Body fat was inversely related with insulin signaling in HFO, but not CFO. These findings suggest that paternal high©\fat feeding contributes to enhanced whole©\body and skeletal muscle insulin sensitivity in HFO early in life; however, these benefits are lost by early adulthood, potentially due to premature increases in body fat
%K Akt
%K AS160
%K diet
%K father
%K insulin sensitivity
%K muscle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827533/