%0 Journal Article
%T Glucocorticoid-resistant B cell acute lymphoblastic leukemia displays receptor tyrosine kinase activation
%A Johan Vallon-Christersson
%A Julhash U. Kazi
%A Kinjal Shah
%A Rohit A. Chougule
%A Sausan A. Moharram
%J Archive of "NPJ Genomic Medicine".
%D 2019
%R 10.1038/s41525-019-0082-y
%X Prolonged dexamethasone treatment induces dexamethasone resistance in B cell acute lymphoblastic leukemia (B-ALL) cells: a human B-ALL cell lines 697, NALM-6, RS4;11, and TANOUE were treated with increasing concentrations (from 1£¿nM to 5£¿¦ÌM) of dexamethasone for 90 days following 14 days of culture in the absence of dexamethasone. Half-maximal effective concentration (EC50) was measured using PrestoBlue cell viability assay. Initial EC50 values were measured before dexamethasone treatment started. b B-ALL cells were lysed and lysates were processed for Western blotting analysis using anti-glucocorticoid receptor (GR) and anti-¦Â-actin antibodies. Blots were derived from the same experiment and were processed in paralle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449402/