%0 Journal Article %T Mucins trigger dispersal of Pseudomonas aeruginosa biofilms %A Gerardo C¨¢rcamo-Oyarce %A Julia Y. Co %A Katharina Ribbeck %A Kelsey M. Wheeler %A Nicole Billings %A Niels Holten-Andersen %A Scott C. Grindy %J Archive of "NPJ Biofilms and Microbiomes". %D 2018 %R 10.1038/s41522-018-0067-0 %X Natively purified mucins MUC5AC and MUC2 trigger the disruption of P. aeruginosa biofilms. a PAO1-GFP biofilms (48£¿h) were exposed to medium with increasing concentrations of mucins. The flow rate of the medium was 0.5£¿¦ÌL/min. At 0.5% (w/v) and above, MUC5AC reduced biofilm mass. Scale bars£¿=£¿20£¿¦Ìm. b Confocal images of biofilms were analyzed using COMSTAT to quantify biomass after 1£¿h of exposure to mucins. Dotted lines indicate average values for 48£¿h biofilms before exposure (t£¿=£¿0£¿h). Error bars represent standard error (n£¿¡Ý£¿3). *P£¿¡Ü£¿0.05, unpaired Student¡¯s t test. c Mucin-mediated biofilm erosion is affected by the flow rate. Quantification of 48£¿h PAO1-GFP biofilm biomass after 1£¿h of exposure to LB with or without 0.5% mucins at slow flow (0.5£¿¦ÌL/min) or fast flow (10£¿¦ÌL/min). Values are normalized to biofilm biomass before exposure. Error bars represent standard error (n£¿¡Ý£¿3). *P£¿¡Ü£¿0.05, unpaired Student¡¯s t test. A comparison between mucin MUC5AC and MUC2 shows that both mucins disassemble P. aeruginosa biofilms. d Live confocal imaging and e biofilm biomass quantification of 48£¿h PAO1-GFP biofilms before (t£¿=£¿0£¿h) and after (t£¿=£¿1£¿h) exposure to medium with or without 0.5% mucins at 0.5£¿¦ÌL/min flow. Scale bars£¿=£¿20£¿¦Ìm. Error bars represent standard error (n£¿=£¿3). *P£¿¡Ü£¿0.05, unpaired Student¡¯s t tes %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180003/