%0 Journal Article
%T Human cell-derived tissue-engineered heart valve with integrated Valsalva sinuses: towards native-like transcatheter pulmonary valve replacements
%A Emanuela S. Fioretta
%A Etem Caliskan
%A H¨¦ctor Rodriguez Cetina Biefer
%A Mareike Sauer
%A Matilde Putti
%A Maximilian Y. Emmert
%A Miriam Lipiski
%A Nikola Cesarovic
%A Petra E. Dijkman
%A Sarah E. Motta
%A Simon P. Hoerstrup
%A Valentina Lintas
%J Archive of "NPJ Regenerative Medicine".
%D 2019
%R 10.1038/s41536-019-0077-4
%X Qualitative evaluation of hTEM patches cultured with hDF and quantitative tissue composition after 4 weeks culture at different TGF-¦Â1 concentrations. a Representative image of the polyglycolic acid/poly-4-hydroxybutyrate scaffold sutured onto a stainless metal ring before in vitro culture. b Representative image of a hTEM after 4 weeks culture showing shiny neo-tissue deposition and homogeneous matrix distribution. c GAGs content showed slightly higher amount in patches cultured at 5£¿ng/ml TGF-¦Â1. d HYP content showed similar expression values among all the different groups. e¨Ch Representative images of H&E staining show more pronounced neo-tissue formation for hTEM cultured with TGF-¦Â1 in comparison with the control group. i¨Cl VGEL staining did not show any expression of elastic fibers at 4 weeks follow-up. Col-1 m¨Cp and Col-3 q¨Ct deposition is enhanced in hTEM cultured in the presence of TGF-¦Â1 (scale bars 100£¿¦Ìm). Dot plot graphs present mean£¿¡À£¿standard deviation (center line and bounds of error bars, respectively
%K Preclinical research
%K Translational research
%K Valvular disease
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572861/