%0 Journal Article
%T Combination of stem cell and gene therapy ameliorates symptoms in Huntington¡¯s disease mice
%A Alvince Learnz Pongos
%A Anthony Wing Sang Chan
%A Carissa Emerson Hunter
%A In Ki Cho
%A Sarah Ye
%J Archive of "NPJ Regenerative Medicine".
%D 2019
%R 10.1038/s41536-019-0066-7
%X Suppression of HTT by expressing shHD. a qRT-PCR analysis of HTT exon 1 expression of HD-shHD-NPC showing significant suppression of HTT in HD-shHD-NPC compared to both HD-NPC (P£¿<£¿0.0001) and WT-NPC (P£¿=£¿0.003). Results shown from three biological replicates. Data is represented as mean£¿¡À£¿SEM (*P£¿<£¿0.05, ***P£¿<£¿0.0001; ANOVA). b Western blot analysis showing increased oligomeric mHTT aggregates in stacking gel (mEM48) and soluble form (1C2) in HD-NPC while expression of shHD in HD-NPC decreased both aggregate accumulation in the stacking gel and soluble form of mHTT. Positive control used in this study was 293FT cells overexpressing expanded poly-Q (84Q) under the control of ubiquitin promote
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435637/