%0 Journal Article
%T Oncogenic KIT mutations induce STAT3-dependent autophagy to support cell proliferation in acute myeloid leukemia
%A Carine Joffre
%A Christian R¨¦cher
%A Cl¨¦ment Larrue
%A Estelle Saland
%A H¨¦l¨¦na Boutzen
%A Jean-Emmanuel Sarry
%A Quentin Heydt
%A Tony Kaoma
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0148-9
%X a¨Cd Oncogenic KITD816V drives autophagy. a TF-1 or TF-1 KITD816V cells were incubated for 4£¿h with PBS, Bafilomycin A1 (20£¿nM, n£¿=£¿3£¿¡À£¿SEM), or chloroquine (20£¿¦ÌM, n£¿=£¿2£¿¡À£¿SD), and were then analyzed by immunoblotting using the appropriate antibodies. Numbers represent the LC3B-II/actin ratios obtained by densitometric analysis. b Cells were incubated for 4 or 8£¿h with vehicle or 20£¿¦ÌM of chloroquine, and then incubated with Cyto-ID before flow cytometry (n£¿=£¿3£¿¡À£¿SEM). ¦¤MFI£¿=£¿MFI(chloroquine)£¿£¿£¿MFI(vehicle). c Cells were treated with vehicle or BafA1 for 2£¿h before LC3 staining, fluorescent labeling, and immunofluorescence analysis. d Quantification of LC3-positive autophagosomes (n£¿=£¿3£¿¡À£¿SEM). e, f Pharmacological inhibition or genetic invalidation of a decrease in KIT autophagic flux. e TF-1 KITD816V cells were treated overnight with PKC412 at 1£¿¦ÌM; 20£¿nM of BafA1 was added at 2£¿h before cell lysis and western blotting. Numbers represent the LC3B-II/actin ratios obtained by densitometric analysis (n£¿=£¿2£¿¡À£¿SD). f KITD816V cells were incubated for 3 days with doxycycline to induce expression of shRNA against KIT. Cells were treated for 2£¿h with BafA1 (20£¿nM) and then analyzed by western blotting. Numbers represent the LC3B-II/actin ratios obtained by densitometric analysis (n£¿=£¿2£¿¡À£¿SD
%K Autophagy
%K Leukaemia
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635375/