%0 Journal Article
%T ZFHX3 is indispensable for ER¦Â to inhibit cell proliferation via MYC downregulation in prostate cancer cells
%A Baotong Zhang
%A Changying Fu
%A Jin-Tang Dong
%A Juan Li
%A Jun A
%A Liya Fu
%A Qingxia Hu
%A Rui Chen
%A Xing Fu
%A Zhiqian Zhang
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0138-y
%X a Expression of ZFHX3, AR, ER¦Á, and ER¦Â in human prostate epithelial cell lines, as determined by Western blotting. Breast cancer cell lines MCF-7 and MDA-MB-231 were used as positive and negative controls, respectively, for ER¦Á. b Treatment with DPN, an ER¦Â activator, decreased cell proliferation in two-dimensional (2D) culture in both C4-2B (left) and LNCaP (right) cell lines. Cells were serum-starved for 48£¿h before indicated DPN treatments. Optical densities represent cell numbers. n£¿=£¿4. c Knockdown of ER¦Â eliminated the inhibitory effect of DPN on colony formation in both C4-2B (left) and LNCaP (right) cell lines in plates pre-coated with 0.35% soft agar. Cells were transfected with siESR2-2, which showed the highest efficiency of knockdown among the three siRNAs against ESR2, for 24£¿h and treated with DPN (0.1£¿¦ÌM) for 2 weeks. Colonies with a diameter >£¿100£¿¦Ìm were counted. Knockdown of ER¦Â was validated by Western blotting. n£¿=£¿3. d Inhibition of ER¦Â function by its antagonist PHTPP eliminated the inhibitory effect of DPN on colony formation. n£¿=£¿3. e DPN upregulates ZFHX3 expression but downregulates MYC and CCND1 in C4-2B and/or LNCaP cells. DPN treatments were at 0, 0.01, 0.1, and 1£¿¦ÌM for 48£¿h, and protein expression was determined by Western blotting. f Inhibition of ER¦Â function by its antagonist PHTPP eliminated the inhibitory effect of DPN on MYC expression in C4-2B cells. DPN and PHTPP were at 0.5£¿¦ÌM. *P£¿<£¿0.05; **P£¿<£¿0.01; ns, not significant. ZFHX3, zinc-finger homeobox 3; AR, androgen receptor; ER¦Á, estrogen receptor alpha; DPN, diarylpropionitrile; siRNA, small interfering RNA; PHTPP, 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]pheno
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461672/