%0 Journal Article
%T Hypomethylation at non-CpG/CpG sites in the promoter of HIF-1¦Á gene combined with enhanced H3K9Ac modification contribute to maintain higher HIF-1¦Á expression in breast cancer
%A Chun Li
%A Junyu Tan
%A Wei Xiong
%A Wenjun Xiao
%A Xiong Liu
%A Yaochen Li
%A Yuxian Guo
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0135-1
%X a Boxplots on the upper row showing relative expression of HIF-1¦Á in basal, HER2 positive, luminal A, luminal B, normal-like, and nonclassified breast cancer patients. Boxplots in the lower-left corner showing relative expression of HIF-1¦Á in ER¦Á positive and negative status. Boxplots in the lower-right corner showing relative expression of HIF-1¦Á in breast cancer patients with grade 1, 2, and 3. b Western blotting analysis of HIF-1¦Á expression in various breast cancer cell lines. c MSP analysis of the methylation status of the HIF-1¦Á promoter. ¡°U¡± indicates unmethylated amplification, and ¡°M¡± indicates methylated amplification. d Western blotting shows changes in the expression levels of HIF-1¦Á and HIF-2¦Á after the cells were treated with RG-108 or 5-Aza of different concentrations. e qRT-PCR shows changes in the expression levels of HIF-1¦Á and HIF-2¦Á after the cells were treated with RG-108 of different concentrations. f qRT-PCR shows changes in the expression levels of HIF-1¦Á and HIF-2¦Á after the cells were treated with 5-Aza of different concentrations. g HIF-1¦Á activities in MCF-7 and MDA-MB-231 cells were tested respectively by co-transfecting 6XHRE reporter vector and a Renilla luciferase plasmid. Data are presented as the means£¿¡À£¿SD of three independent experiments. *p£¿<£¿.05; **p£¿<£¿.01; and ***p£¿<£¿.001 (Student¡¯s t-test) as compared to control cell
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445832/