%0 Journal Article
%T The CSN3 subunit of the COP9 signalosome interacts with the HD region of Sos1 regulating stability of this GEF protein
%A Alberto Fern¨¢ndez-Medarde
%A Alicia Ballester
%A Ana Bel¨¦n C¨¢mara
%A Bego£¿a Anta
%A Berta Anta
%A Eugenio Santos
%A Jos¨¦ Lu¨ªs Oliva
%A Jos¨¦ M. Rojas-Caba£¿eros
%A Mar¨ªa Pilar de Lucas
%A Natasha Zarich
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-018-0111-1
%X a Scheme of the primary structure of the truncated fragments of hSos1. b The constructs in pEG202 coding respectively for the LexA fused to the HD or NDP and vector pJG4-5 alone (without CSN3) do not present ¦Â-galactosidase activity in X-Gal plates assays. Three independent colonies from each co-transformation were analyzed. c B42-activation domain fusion plasmid coding for B42-CSN3 (construct in pJG4-5) was co-transformed into yeast strain EGY48 (ura3, his3, trp1, LexAop-leu2) with the plasmid pSH18-34 (LacZ reporter with LexA-binding sites) and with LexA DNA binding domain fusion plasmids (constructs in pEG202) coding respectively for the LexA fused to the same HD, DH, PH, DH-PH, NDP fragments and for the PRII region of hSos1 (negative control). As others negative controls, plasmid pEG202 was co-transformed with pJG4-5-CSN3 (Vector£¿+£¿CSN3). Three independent colonies from each co-transformation were analyzed for ¦Â-galactosidase activity in color X-Gal plates assays. The specificity of the interactions was tested by dependence to galactose-raffinose. d LEU+ phenotype by patching these colonies in medium lacking leucin
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328564/