%0 Journal Article
%T Cullin 5 is a novel candidate tumor suppressor in renal cell carcinoma involved in the maintenance of genome stability
%A Anette Duensing
%A Claudia Scholl
%A Markus Hohenfellner
%A Mar¨ªa ¨¢ngeles Tapia-Laliena
%A Nina Korzeniewski
%A Samuel Pe£¿a-Llopis
%A Stefan Duensing
%A Stefan Fr£¿hling
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-018-0110-2
%X a (upper panel) Quantification of cells with >4 centrioles following siRNA-mediated depletion of human cullin subunits in U-2 OS/centrin-GFP cells (72£¿h). Each bar represents mean plus standard error of at least two independent experiments with a minimum of 300 cells counted per experiment. Asterisks indicate statistically significant differences (*p£¿¡Ü£¿0.05; **p£¿¡Ü 0.005). a (lower panel) Fluorescence microscopic analysis of U-2OS cells stably expressing centrin-GFP and transiently transfected with control siRNA or siRNA duplexes targeting CUL5. Note the presence of supernumerary daughter centrioles after CUL5 knock-down as indicated by the weaker GFP signals. b Fluorescence microscopic analysis (left panels) and quantification (right panel) of U-2 OS/centrin-GFP cells transfected with either DN-CUL5 or a control plasmid (48£¿h). dsRed was used as transfection marker. Arrows indicate daughter centrioles. Scale bar£¿=£¿10£¿¦Ìm. Mean and standard error of three independent experiments with at least 100 cells counted per experiment are shown. c Immunofluorescence microscopic analysis of U-2 OS/centrin-GFP cells for CUL5. Note the association of CUL5 with the bigger one of the two unduplicated centrioles (arrow, top panels) and with the second centriole in cells with duplicated centrioles (arrows, bottom panels). Scale bar£¿=£¿10£¿¦Ìm. d Co-immunofluorescence microscopic analysis of U-2 OS/centrin-GFP cells for CUL5 and CEP170. Note the enhanced co-localization of CUL5 with CEP170 at the older, mature centriole. Arrowheads point to the areas shown in insets. e Immunofluorescence microscopic analysis (left panels) and quantification (right panel) of U-2 OS centrin/GFP cells with centriole overduplication in the presence of 1 or 2 or >2 CEP170-positive centrioles following siRNA-mediated depletion of CUL5 (72£¿h). Scale bar£¿=£¿10£¿¦Ì
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328621/