%0 Journal Article %T IKK¦Â activates p53 to promote cancer cell adaptation to glutamine deprivation %A Mari B. Ishak Gabra %A Mei Kong %A Michael A. Reid %A Thai Q. Tran %A Xazmin H. Lowman %A Ying Yang %J Archive of "Oncogenesis". %D 2018 %R 10.1038/s41389-018-0104-0 %X a HT1080 transduced with control (Ctrl) or IKK¦Â shRNA were cultured in glutamine-free medium for 24 and 48£¿h. Cell viability was assessed by PI exclusion. Cell lysate was collected for western blotting with the indicated antibodies. b Western blot analysis of wild-type (WT), Ikk¦Á£¿/£¿ or Ikk¦Â£¿/£¿ MEFs and HT1080 cells transiently transfected with siRNA against scrambled control, IKK¦Á, or IKK¦Â cultured in complete and glutamine (Gln)-free medium 24h and cell lysate was used for immunoblotting using the antibodies indicated. c Wild-type and Ikk¦Â£¿/£¿ MEFs were cultured in complete (Comp) or glutamine-free (No Gln) media overnight and mRNA was extracted for qPCR analysis of p53-target genes. d HT1080 cells transiently transfected with siRNA against scrambled control or IKK¦Â were cultured in complete or glutamine-free media overnight. mRNA was extracted, and expression of p53-target genes was determined using qPCR. a, c, and d represent means£¿¡À£¿s.d. of triplicates from three independent experiments (*p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.001 using Student¡¯s t-test %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255781/