%0 Journal Article
%T MEK inhibitors induce apoptosis via FoxO3a-dependent PUMA induction in colorectal cancer cells
%A Dapeng Ding
%A Lin Lin
%A Shijun Li
%A Yan Li
%A Yanmei Jiang
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0078-y
%X a The indicated cell lines were treated with increasing doses of trametinib or selumetinib for 72£¿h. Cell proliferation was determined by MTS assay. b The indicated cell lines were treated with 0.1£¿¦̀mol/L trametinib or 0.1£¿¦̀mol/L selumetinib for 24£¿h at the indicated concentrations. Apoptosis was analyzed by Annexin V/PI staining followed by flow cytometry. c The indicated cell lines were treated with 0.1£¿¦̀mol/L trametinib or 0.1£¿¦̀mol/L selumetinib for 24£¿h at the indicated concentrations. Caspase 3/7 activity was determined by fluorogenic analysis. d The indicated cell lines were treated with 0.1£¿¦̀mol/L trametinib or 0.1£¿¦̀mol/L selumetinib at the indicated time points. Cleaved caspase 3, 8, and 9 were analyzed by Western blotting. The results in a, b, and c are expressed as the means£¿¡À£¿SD of three independent experiments. **P£¿<£¿0.01, *P£¿<£¿0.0
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127344/