%0 Journal Article
%T FOXC1 plays a crucial role in the growth of pancreatic cancer
%A Adriana Galvez
%A Alejandro Rodriguez
%A Alexa Clift
%A Animesh Chatterjee
%A Carly Ivy Levin
%A Diego A. Pedroza
%A Fernando A. Camacho
%A Joshua Dodderer
%A Kristina Flores
%A Mauricio Terres
%A Megan Miranda
%A Rajkumar Lakshmanaswamy
%A Ramadevi Subramani
%A Sai Navana Kolli
%A Servando Rivera
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0061-7
%X a Activity of FOXC1 transcription factor in IGF-1R-silenced HPAC cells. b Relative mRNA expression level of FOXC1 was assessed by real-time RT-PCR in IGF-1R-silenced HPAC cells. c Western blot analysis of IGF-1R and FOXC1 expression in IGF-1R-silenced HPAC cells. d Representative photographs of immunofluorescence analysis of FOXC1 expression in IGF-1R-silenced HPAC cells were visualized via confocal microscopy at ¡Á£¿60 magnification. e Representative qualitative (FOXC1-positive cells indicated by brown staining) and quantitative immunohistochemical analysis (bar diagram represents # of FOXC1-positive cells/field) of FOXC1 by tumor stage in human pancreatic ductal adenocarcinoma tissues and in normal pancreas tissue at ¡Á£¿40 magnification. IHC staining was quantitated at five random fields per section with at least 200 cells/field. f Western blot analysis of FOXC1 expression in a panel pancreatic cancer cell lines (HPAC, Capan-2, Capan-1, AsPC-1, PANC-1, MIA PaCa-2, and BxPC-3). g HPAC cells were transfected with three predesigned FOXC1 siRNAs (labeled as A, B, and C) at concentrations of 20, 40, and 60£¿nM along with scrambled control siRNA (Scr). Silencing efficacy of FOXC1 siRNA was determined using western blot analysis. h Representative photographs of immunofluorescence analysis of FOXC1 expression in FOXC1-silenced HPAC cells at ¡Á£¿60 magnification. i Relative mRNA expression level of FOXC1 was assessed by real-time PCR in FOXC1-silenced HPAC cells. j MIA PaCa-2 cells were transfected with FOXC1-overexpressing plasmid DNA from three different colonies (labeled as C1, C2, and C3) at concentrations of 1, 5, and 10£¿¦Ìg along with pCMV control plasmid DNA. Overexpression efficacy of FOXC1 plasmid DNA in MIA PaCa-2 cells was determined using western blotting. k Representative photographs of immunofluorescence analysis of FOXC1 expression in FOXC1-overexpressing MIA PaCa-2 cells at ¡Á£¿60 magnification. l Relative mRNA expression level of FOXC1 was assessed by real-time PCR in FOXC1-overexpressing MIA PaCa-2 cells. Data shown as mean£¿¡À£¿SEM. Experiments (n£¿=£¿3) were repeated three times in triplicates. *p£¿<£¿0.0
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033944/