%0 Journal Article
%T Effects of Triton X-100 and PEG on the Catalytic Properties and Thermal Stability of Lipase from Candida Rugosa Free and Immobilized on Glyoxyl-Agarose
%A Ang¨¦lica M.S. Vieira
%A Cleide M.F. Soares
%A Gisella M Zanin
%A Let¨ªcia M. Sgobi
%A Marcelo F. Vieira
%A Paulo W. Tardioli
%A Poliana C. Tiosso
%A Rafael F. Perna
%J Archive of "The Open Biochemistry Journal".
%D 2017
%R 10.2174/1874091X01711010066
%X Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose prepared in bicarbonate buffer, pH 10. This change of protocol was enough for allowing the preparation of derivatives actives of CRL on glyoxyl-agarose and verifying the effect of this modified procedure on the properties of the immobilized enzyme. The effect of the additives Triton-X-100 and polyethylene glycol (PEG) on the enzymatic activity recovery and immobilized enzyme stability was evaluated
%K Triton X-100
%K PEG
%K Stabilization
%K Candida rugosa Lipase
%K Immobilization
%K Glyoxyl-Agarose
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721316/