%0 Journal Article %T Effects of Triton X-100 and PEG on the Catalytic Properties and Thermal Stability of Lipase from Candida Rugosa Free and Immobilized on Glyoxyl-Agarose %A Ang¨¦lica M.S. Vieira %A Cleide M.F. Soares %A Gisella M Zanin %A Let¨ªcia M. Sgobi %A Marcelo F. Vieira %A Paulo W. Tardioli %A Poliana C. Tiosso %A Rafael F. Perna %J Archive of "The Open Biochemistry Journal". %D 2017 %R 10.2174/1874091X01711010066 %X Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose prepared in bicarbonate buffer, pH 10. This change of protocol was enough for allowing the preparation of derivatives actives of CRL on glyoxyl-agarose and verifying the effect of this modified procedure on the properties of the immobilized enzyme. The effect of the additives Triton-X-100 and polyethylene glycol (PEG) on the enzymatic activity recovery and immobilized enzyme stability was evaluated %K Triton X-100 %K PEG %K Stabilization %K Candida rugosa Lipase %K Immobilization %K Glyoxyl-Agarose %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721316/