%0 Journal Article
%T Platelet-derived growth factor receptor-¦Á and -¦Â promote cancer stem cell phenotypes in sarcomas
%A Brendan C. Yi
%A Changhwan Yoon
%A Kevin K. Chang
%A M. Celeste Simon
%A Sam S. Yoon
%A William D. Tap
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0059-1
%X a Western blot analysis of cell surface markers for cancer stem-like cells in human sarcoma cell lines HT1080, SK-LMS-1, and DDLS8817. Cells were grown as monolayers or spheroids. ¦Â-actin was used as the loading control. b Percentage of CD133(+) cells in human sarcoma cells grown as monolayers or as spheroids as determined by FACS analysis. *p£¿<£¿0.05 compared to Monolayers. c Western blot analysis of human sarcoma cells grown as monolayers or as spheroids for self-renewal transcription factors. d Western blot analysis of FACS-sorted CD133(+) and CD133(£¿) spheroid cells for self-renewal transcription factors. Experiments in a¨Cd were performed three times with similar results. e Representative light microscopy images from single cell assays performed in triplicate for human sarcoma spheroid cells FACS-sorted by CD133. Graph displays the mean diameters of the spheroid colonies. Bars represent standard deviation. *p£¿<£¿0.05 compared to CD133(£¿
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006341/