%0 Journal Article
%T High-density lipoprotein ameliorates palmitic acid-induced lipotoxicity and oxidative dysfunction in H9c2 cardiomyoblast cells via ROS suppression
%A Chang-Hai Tsai
%A Chih-Hsin Tang
%A Chih-Yang Huang
%A Fuu-Jen Tsai
%A Jai-Sing Yang
%A Jing-Gung Chung
%A Kuen-Ming Wu
%A Li-Yi Cheng
%A Mei-Chin Ying
%A Po-Hua Su
%A Vijaya Padma Viswanadha
%A Wei-Wen Kuo
%A Yuan-Man Hsu
%J Archive of "Nutrition & Metabolism".
%D 2019
%R 10.1186/s12986-019-0356-5
%X PA increased oxidative stress and induced cell death in H9c2 cells. a H9c2 cells were treated with PA at different concentrations for 24 or 48£¿h. Cells viability was measured by MTT assay. b H9c2 cells were incubated with PA (0.5£¿mM) for 24£¿h. Cells were stained with 4,6-diamidino-2-phenylindole (DAPI) and terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) assay. H9c2 cells were treated with PA (0.5£¿mM) for 24£¿h followed by 1£¿h incubation with fluorescent probe (c) DCF-AM (10£¿¦ÌM) (d) DHE (10£¿¦ÌM) (e) MitoSOX£¿ (5£¿¦ÌM). Fluorescence intensity of cells was measured by flow cytometry. H9c2 cells were treated with PA (0.5£¿mM) for indicated time. f The levels of NADPH oxidase (Nox2-gp91, p47phox, Rac) and (g) antioxidant enzymes (SOD1, SOD2) were measured by Western blot. Data showed the means ¡À SEM of 3 independent analyses.*p£¿<£¿0.05 and **p£¿<£¿0.01 compared with the contro
%K High-density lipoprotein
%K Palmitic acid
%K Lipotoxicity
%K Cardiomyoblast
%K ROS
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537189/