%0 Journal Article
%T Effects of sildenafil treatment on thermogenesis and glucose homeostasis in diet-induced obese mice
%A Beate Herrmann
%A Jens Mittag
%A Kornelia Johann
%A Lisbeth Harder
%A Marlen Colleen Reis
%A Rebecca Oelkrug
%A Sogol Gachkar
%J Archive of "Nutrition & Diabetes".
%D 2018
%R 10.1038/s41387-018-0026-0
%X a Eight days of sildenafil treatment induced a significant elevation of hepatic cGMP levels in DIO mice (P£¿=£¿0.0089). b However, body weight of control mice and mice treated with sildenafil was not affected by the treatment and c organ weights of iBAT, iWAT, eWAT, and liver (normalized to body weight) were similar. d Rectal body temperature was measured using a small-diameter thermocouple probe (accuracy: 0.1£¿¡ãC) before (day 0) and during the sildenafil treatment (day 5). e¨Cf In parallel, infrared thermography (sensitivity: 0.05£¿¡ãC) of the iBAT area was performed on conscious animals and both were unchanged after 5 days of treatment. g¨Ci In line, gene expression analysis in iBAT revealed no differences in expression of Ucp1, Ppar¦Ã, Fabp4, Cd36, Cd68, and Mcp-1, whereas in iWAT Ppar¦Ã (P£¿=£¿0.0233) and Fabp4 (P£¿=£¿0.0425) were significantly downregulated and Cd68 (P£¿=£¿0.0201) upregulated. Furthermore, Mcp-1 expression was significantly lower in eWAT after sildenafil treatment (P£¿=£¿0.0406). *P£¿<£¿0.05, **P£¿<£¿0.01, unpaired, two-tailed t-tests with Welch-correction (a, c, g¨Ci). Repeated measures two-way ANOVA following Bonferroni¡¯s multiple comparisons test (b, d¨Cf). Data are presented as mean£¿¡À£¿s.e.m., n£¿=£¿8. Cd36 cluster of differentiation 36, Cd68 cluster of differentiation 68, cGMP cyclic guanosine monophosphate, eWAT epididymal white adipose tissue. Fabp4 fatty acid-binding protein 4, iBAT interscapular brown adipose tissue, iWAT inguinal white adipose tissue, Lep Leptin, Mcp-1 monocyte chemoattractant protein-1, Ppar¦Ã peroxisome proliferator-activated receptor gamma, Ucp1 uncoupling protein
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856821/