%0 Journal Article
%T Single-cell analysis reveals individual spore responses to simulated space vacuum
%A Lin He
%A Marta Cortes£¿o
%A Muying Wu
%A Peter Setlow
%A Ralf Moeller
%A Shiwei Wang
%A Yong-qing Li
%J Archive of "NPJ Microgravity".
%D 2018
%R 10.1038/s41526-018-0059-7
%X Viability, DPA levels and Raman spectra of spores with and without high vacuum treatment. a, b Viability of B. subtilis PS533 and NCIB 3610 spores that were exposed to high vacuum (<2.6£¿¡Á£¿10£¿5£¿Pa) for various periods. Spores were first dried in a centrifuge tube and were exposed to high vacuum for 1 or 10 days. Spores that were dried in ambient laboratory pressure but not exposed to high vacuum are the control spores. After high vacuum exposure, spores were suspended in 100£¿uL distilled water and cultured on LB plates at 37£¿¡ãC, and spore viability was normalized to that of the control spores. c, d Average Raman spectra of multiple single spores of B. subtilis PS832 c and B. cereus d at atmospheric pressure (1.0£¿¡Á£¿105£¿Pa) (control) and high vacuum. The spectra of spores during the exposure to high vacuum were directly measured using image-guided micro-Raman spectroscopy. The laser power was 20£¿mW and integration time was 30£¿s, and spectra shown were averaged over 30 individual spores. *P£¿<£¿0.05%; ns, no significanc
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279783/