%0 Journal Article
%T Micro-CT X-ray imaging exposes structured diffusion barriers within biofilms
%A Alona Keren-Paz
%A Ilana Kolodkin-Gal
%A Vlad Brumfeld
%A Yaara Oppenheimer-Shaanan
%J Archive of "NPJ Biofilms and Microbiomes".
%D 2018
%R 10.1038/s41522-018-0051-8
%X Imaging and quantifying calcium-rich structures in B. subtilis colonies. B. subtilis and M. smegmatis colonies grown on 1.5% B4 agar, supplemented with Ca acetate, as indicated in the main text. a Left panel¡ª3D reconstruction. Middle panel¡ªsegmentation of the reconstructed volume, red indicates the densest mineral. Right panel¡ªtransversal slices. Scale bar: 200£¿¦Ìm. b Relative volume of the mineral layer of the total B. subtilis colony volume. Averages of three independent experiments are displayed, and the bars represent standard deviation. c TGA analysis of calcium minerals in B. subtilis colonies. Averages of three independent experiments are displayed, and the bars represent standard deviation. d Estimated thickness of the mineral layer in B. subtilis colony. Averages of three independent experiments are displayed, and the bars represent standard deviatio
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904145/