%0 Journal Article
%T Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance
%A D. S. Guttman
%A P. J. Stapleton
%A P. W. Wang
%A T. Beaudoin
%A V. Waters
%A Y. C. W. Yau
%A Y. Gong
%J Archive of "NPJ Biofilms and Microbiomes".
%D 2017
%R 10.1038/s41522-017-0035-0
%X Staphylococcus aureus filtrates (SaF) do not affect initial attachment of Pseudomonas aeruginosa to borosilicate slide chambers. a Schematic representation of how SaF biofilm formation was assessed. P. aeruginosa was grown overnight in liquid culture prior to seeding in 8-well slide chambers. After 6£¿h of attachment, media was removed and replaced with fresh media. b Representative images of P. aeruginosa biofilms grown for 24£¿h in the presence of 10% (v/v) SaF in LB. Green cells: live; red cells: dead. c¨Cf Image analysis of biofilm thickness c, surface coverage d, biomass e and % dead cells f for given biofilms. Biofilms were grown as described above in the presence of LB alone (black bars) or 10%SaF (white bars) prior to image acquisition and analysis with COMSTAT. N£¿=£¿7 eradicated isolates and N£¿=£¿7 persistent isolates. The mean of PA01 was generated from seven biological replicates. All means are plotted with standard deviation. Statistics were performed using non-parametric one-way ANOVA (Kruskal¨CWallis) with Dunn¡¯s post-test for multiple comparison
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648753/