%0 Journal Article
%T Model-based identification of TNF汐-induced IKK汕-mediated and I百B汐-mediated regulation of NF百B signal transduction as a tool to quantify the impact of drug-induced liver injury compounds
%A Amy Schofield
%A Angela Oppelt
%A B. Kevin Park
%A Benjamin Merkt
%A Bob van de Water
%A Carmen L. Kr邦ger
%A Christopher E. Goldring
%A Daniel Kaschek
%A Fang Zhang
%A Franziska Herrmann
%A Hassan Malik
%A Jens Timmer
%A Lea Zimmermann
%A Mara Meub
%A Marcel Schilling
%A Marie Buck-Wiese
%A Mike Heilemann
%A Robert P. Jones
%A Rowena Sison-Young
%A Sebastian Malkusch
%A Suzanna Huppelschoten
%A Ursula Klingm邦ller
%J Archive of "NPJ Systems Biology and Applications".
%D 2018
%R 10.1038/s41540-018-0058-z
%X TNF汐-induced NF百B signaling pathway model for HepG2 cells and primary human hepatocytes (PHH). a Schematic representation of the model according to Systems Biology Graphical Notation indicating the considered components and reactions. Arrows indicate biochemical reactions. Boxes with round corners symbolize proteins, parallelograms represent RNA. Slashed circles represent degradation or production. Experimental data was acquired by either live-cell fluorescence microscopy (b) of NF百B translocation (cytoplasm and nuclei, nˋ=ˋ3) and of A20 expression dynamics (nˋ=ˋ1) in HepG2 cells stably expressing NF百B-GFP or A20-GFP, respectively, or quantitative immunoblotting (c每e) of cytoplasmic lysates of HepG2 cells (c, nˋ=ˋ3每6, raw data in Figs. S8每S17) and PHHs (d, nˋ=ˋ3 donors, raw data in Figs. S18每S21) or immunoprecipitations (e, nˋ=ˋ1每3, raw data in Figs. S22每S29) treated with TNF汐 (10ˋng/ml). Data points with 1考 confidence intervals computed from replicates are indicated by dots with error bars, lines indicate trajectories of the calibrated mode
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995845/