%0 Journal Article
%T Construction and Immunological Evaluation of CpG-Au@HBc Virus-Like Nanoparticles as a Potential Vaccine
%A Guohong Zhuang
%A Lei Ren
%A Ning Kang
%A Shengli Bi
%A Wenjun Shan
%A Yarun Wang
%A Yongliang Liu
%A Yue Wang
%J Archive of "Nanoscale Research Letters".
%D 2016
%R 10.1186/s11671-016-1554-y
%X Different types of vaccines have been developed to elicit active immunization to treat various diseases, while suffer from limitation of efficacy. Herein, a novel immunostimulatory nanocomposite (CpG-Au@HBc VLP) was rationally designed by self-assembling engineered virus-like particles encapsulating CpG-gold nanoparticle conjugates through electrostatic interactions. The monodispersed and uniformly sized CpG-Au@HBc VLP showed increased CD4+, CD8+ T cell numbers and stronger secretion of cytokine interferon-gamma than HBc VLPs adjuvanted with conventional Freund¡¯s adjuvant. Furthermore, the use of Au nanoparticles also generated enhanced immunogenicity of CpG and VLPs on both humoral and cellular immune pathways, as followed from increased expressions of total HBc-specific antibody titer, CD4+ T cells, CD8+ T cells, cytokine interleukin-4, and interferon-gamma. These findings demonstrated that CpG-Au@HBc VLP nanocomposite could induce robust cellular and humoral immune response, which could be a potential vaccine for future prophylactic and therapeutic application
%K Virus-like nanoparticles
%K CpG ODNs
%K Gold nanoparticles
%K Immune response
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951386/