%0 Journal Article
%T Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion
%A Bin Li
%A Ci Yin
%A Fu-Rong Wang
%A Hai-Ming Feng
%A Jun-Ping Lin
%A Tao Jing
%A Tie-Niu Song
%A Yu-Qi Meng
%A Zheng Li
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0126-2
%X a Gating strategy for flow cytometry to enumerate macrophages infiltrating ESCC. Debris and doublets were excluded, followed by quantification of TAMs as CD45+£¿Hoechst£¿ F4/80+£¿CD11b+. b PD1 level estimation in TAMs from ESCC tissues. Representative graphs of PD1 levels in TAMs are shown. c Associations of PD1+ TAMs with ESCC patients¡¯ TNM stage are shown. d Immunofluorescence analysis of PD1 (green) and CD68 (red) expression levels in ESCC tissue. e Association of tumor PD1+ TAMs with patient survival. *p£¿<£¿0.01, **p£¿<£¿0.001 (Student¡¯s t-test
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386749/