%0 Journal Article
%T The Immunogenicity of the Tumor-Associated Antigen ¦Á-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain
%A Lucile Tran
%A Jean-Paul Judor
%A Vanessa Gauttier
%A Michel Geist
%A Chantal Hoffman
%A Ronald Rooke
%A Georges Vassaux
%A Sophie Conchon
%J Journal of Biomedicine and Biotechnology
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/878657
%X Aim. To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA) vector to induce an immune response against a well-tolerated self-antigen. Methods. rMVA vectors expressing different form of α-fetoprotein (AFP) were produced and characterized. Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membrane-bound, or an intracellular form. The immune response was monitored by an IFNΓ ELISpot assay and antibody detection. Results. Vaccination with the membrane-associated form of AFP induced a stronger CD8
%U http://www.hindawi.com/journals/biomed/2012/878657/