%0 Journal Article
%T Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes
%A Gang Chen
%A Jing-fei Zhong
%A Wen-Feng Su
%A Xiao-Song Gu
%A Yun Gu
%A Yun-Tian Shen
%A Zi-Han Jin
%J Archive of "Neuroscience Bulletin".
%D 2016
%R 10.1007/s12264-016-0045-6
%X Co-localization of Rab27b with PLP and Cathepsin D in Cultured Oligodendrocytes and in Brain Slices. A Double staining for galactocerebroside (Galc+, mature oligodendrocyte marker, green) and Hoechst (nuclear marker, blue) shows that most Hoechst+ cells also expressed Galc-IR. Scale bar, 25 ¦Ìm. B, C Co-immunostaining for PLP, Rab27b, and cathepsin D reveals co-localization of Rab27b with PLP in late endosomes/lysosomes of cultured oligodendrocytes (B) and in rat brain slices (C). Confocal planes are shown; lower panels are enlarged images from upper panels. Scale bars, 25 ¦Ìm (upper panels) and 5 ¦Ìm (lower panels). D, E Summary of the co-localization of PLP with Rab27b or cathepsin D in cells (n = 5) (D) and slices (n = 5) (E). Error bars indicate SEM
%K Myelination
%K Neuron-glial interaction
%K Oligodendrocyte
%K Lysosome
%K Rab27b
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563785/