%0 Journal Article
%T MEMOIR: A Novel System for Neural Lineage Tracing
%A Jianhong Zhu
%A Zhifu Wang
%J Archive of "Neuroscience Bulletin".
%D 2017
%R 10.1007/s12264-017-0161-y
%X Principle of MEMOIR system. A In MEMOIR system, three types of elements are integrated into the genome, including a Cas9 variant, a set of barcoded scratchpads and a specifically inducible gRNA targeting the scratchpad. Barcoded scratchpads can be collapsed irreversibly by Cas9/gRNA. An inducible degron modulates Cas9 activity. Inverted black triangles in (A) indicate piggyBac terminal repeats. B Schematic of the MEMOIR working in cells: During cell proliferation, stochastic collapsed scratchpads accumulate gradually. Subsequently, single mRNA molecules can be tested with scratchpad-specific probes (green, inset), and a set of barcode-specific probes (purple, pink, inset) through sequential circles of hybridization and imaging
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725379/