%0 Journal Article
%T Implication of 4E-BP1 protein dephosphorylation and accumulation in pancreatic cancer cell death induced by combined gemcitabine and TRAIL
%A Androulla Elia
%A Catherine Marboeuf
%A Charlotte Adiseshiah
%A Michael J Clemens
%A Rebecca J Buckley
%A Ricky Henry-Grant
%A Satvinder Mudan
%A St谷phane Pyronnet
%J Archive of "Cell Death & Disease".
%D 2017
%R 10.1038/s41419-017-0001-z
%X a Sensitivity of cells to gemcitabine was assessed by MTT assay. Cells were treated with increasing amounts of gemcitabine (0.001每1000ˋ米M) for 24ˋh (nˋ=ˋ4). b Sensitivity of cells to TRAIL was assessed by MTT assay. Cells were treated with increasing amounts of TRAIL (0.001每1000ˋng/ml) for 4ˋh (nˋ=ˋ4). c每e Sensitivity of cells to gemcitabine and TRAIL combination treatment was assessed by MTT assay. Cells were treated with increasing amounts of gemcitabine (0.1每100ˋ米M) for 24ˋh (nˋ=ˋ4) and/or 10 or 100ˋng/ml TRAIL for 4ˋh for BxPC-3 and MIA PaCa-2 cells and 6ˋh for PANC-1 cells (nˋ=ˋ4). All experiments were repeated three times and data are provided as meansˋ㊣ˋSEM (one representative experiment is shown). P-values were calculated using Student＊s t-test to determine the statistical significance of the difference between a, b untreated cells and cells treated with either 1000ˋ米M gemcitabine or 1000ˋng/ml TRAIL, respectively (ns: Pˋ>ˋ0.05, *Pˋ<ˋ0.05, **Pˋ<ˋ0.01) and c每e cells treated with 100ˋ米M gemcitabine and cells treated with 100ˋ米M gemcitabine plus 100ˋng/ml TRAIL (***Pˋ<ˋ0.001
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870593/