%0 Journal Article
%T MicroRNA-3619-5p suppresses bladder carcinoma progression by directly targeting ¦Â-catenin and CDK2 and activating p21
%A Chenghe Wang
%A Chuanchang Li
%A Hua Xu
%A Kai Cui
%A Mengyang Zhang
%A Qingsong Zhang
%A Shuo Miao
%A Tao Xiong
%A Xihong Han
%A Zhong Chen
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0986-y
%X a P21 mRNA and miR-3619 expression were reduced in 5637, T24, EJ, and J82 cell lines compared with primary normal human bladder epithelial cells (SV-HUC-1) (*P£¿<£¿0.05 and **P£¿<£¿0.01). b P21 protein expression in BCa cells was assessed by western blot analysis. GAPDH served as a loading control. c P21 protein expression in BCa tissues was detected by IHC assay (400¡Á). d P21 mRNA and miR-3619 expression were reduced in human BCa tissues compared with their expression in adjacent normal tissues. e Kaplan£¿Meier curves and the log-rank test showed that high p21 expression was associated with a favorable OS compared with low p21 expression (P£¿=£¿0.027). f Kaplan£¿Meier curves and the log-rank test indicated that patients with low miR-3619 and p21 expression levels had significantly shorter survival than those with high miR-3619 or p21 expression levels (P£¿=£¿0.013). g, h ¦Â-catenin and CDK2 protein levels were upregulated in BCa cells and tissues compared with normal bladder mucosal epithelial cells and para-cancerous tissue respectively. i ¦Â-catenin and CDK2 protein expression in BCa tissues was detected by IHC assay (400¡Á
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147790/