%0 Journal Article
%T Spontaneously opening GABAA receptors play a significant role in neuronal signal filtering and integration
%A Nathanael O¡¯Neill
%A Sergiy Sylantyev
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0856-7
%X a Example traces of whole-cell continuous recordings from DGCs. Top: Full experimental trace; application of SR (25£¿¦ÌM) blocks GABA-dependent phasic conductance, whereas subsequent application of SR£¿+£¿PTX (50£¿¦ÌM) blocks tonic conductance controlled by s-GABAARs. Horizontal dashed lines mark time intervals over which Itonic was averaged for SR and SR£¿+£¿PTX. Medium: Expanded section of the recording demonstrates the effect of s-GABAARs blocked by PTX. Shaded area shows the amount of tonic current controlled by s-GABAARs. Bottom: Expanded 0.5-s recording interval before application of SR. Shaded area shows the amount of inhibitory current transferred with GABA-induced phasic events. b Statistical summary on effects of s-GABAARs and GABA-dependent effects. Top: SR and SR£¿+£¿PTX decrease RMS noise; bars represent change (¦¤RMS) from RMS in control (for SR) and from RMS under SR (for SR£¿+£¿PTX), asterisks indicate significance of difference from zero; n£¿=£¿6, Student¡¯s paired t test. Medium: Shifts of Ihold induced by the application of SR and SR£¿+£¿PTX; ¦¤Ihold for SR and SR£¿+£¿PTX was calculated similarly to ¦¤RMS. Asterisks indicate significance of difference from zero; n£¿=£¿6, Student¡¯s paired t test. Bottom: Input into inhibitory charge transfer delivered by GABA-dependent GABAARs (phasic events suppressed by SR) and s-GABAARs (tonic response suppressed by SR£¿+£¿PTX). n£¿=£¿6, Student¡¯s paired t test. Colour codes apply for all three bar charts, **P£¿<£¿0.01, ***P£¿<£¿0.00
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057890/